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hammerhead ribozyme (hh)  (Sangon Biotech)

 
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    Sangon Biotech hammerhead ribozyme (hh)
    Hammerhead Ribozyme (Hh), supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hammerhead ribozyme (hh)/product/Sangon Biotech
    Average 90 stars, based on 1 article reviews
    hammerhead ribozyme (hh) - by Bioz Stars, 2026-05
    90/100 stars

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    Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with ribozymes were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.

    Journal: Scientific Reports

    Article Title: Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells

    doi: 10.1038/s41598-024-55088-4

    Figure Lengend Snippet: Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with ribozymes were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.

    Article Snippet: The Hepatitis Delta Virus (HDV) and Hammerhead (HH) ribozymes were amplified from Addgene plasmid #86197, which was a gift from Tang et al ., and similarly cloned to pGEM-T Easy (Promega).

    Techniques: Expressing, Mutagenesis